Japanese scientists have grown "blanks" of the liver from stem cells
Japanese biologists have created a technique that allows you to grow high-grade "feed" the liver from reprogrammed stem cells and transplant them into the body of the mouse or human, which will in the near future to recover these bodies at damage and disease, according to a paper published in the journal Nature .
RIA Novosti reported that in June 2012 the Japanese biologists led by Hideki Taniguchi (Hideki Taniguchi) from the University of Yokohama (Japan) took a big step towards the creation of an artificial liver, learning to transform the reprogrammed stem (iPS) cells in isolated hepatocytes, the major cells of the liver .
In his new job Taniguchi and his colleagues created a "blank" liver successfully transplanted them into the body of the mouse, and turned them into something like this body. To do this, scientists have tracked for what is happening in the development of the liver in the human fetus and replicated these conditions in a Petri dish with future hepatocytes. In particular, they are added to the culture of the particular cells of vascular walls, umbilical cord as well as cells "blank" for future vascular walls liver.
After 48 hours the cultures radically transformed - they are compressed to small lumps resembling prototype of the liver bud. The similarity was not only outside - these "buds" as they were dubbed by biologists, were active those genes that are commonly associated with the work of hepatocytes.
To test the viability of its scientists transplanted the "buds" under the skin of mice with disabled immune system and monitor their growth. On the second day after implantation of the workpiece "attached" to the circulatory system of the host and began to grow. Two months later, scientists have full authority exercising all the functions of the liver.
According to Taniguchi and his colleagues, artificial liver was stable and was not exposed to mutations in the months of the experiment. This fact, according to biologists, allows you to start work on the adaptation of this method of cultivation of the liver in humans.
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